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bcl2fastq

Conversion software that demultiplexes sequencing data and converts base call (BCL) files into FASTQ files


bcl2fastq requires an environment module

In order to use bcl2fastq, you must first load the appropriate environment module:

module load gnu bcl2fastq

The bcl2fastq program is a data converter for bioinformatics data produced by Illumina sequencers. It is able to convert BCL formatted sequence data to FASTQ sequence data.

Using bcl2fastq on the HPC#

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# Load environment modules
$ module load gnu bcl2fastq

# Execute the command  
$ bcl2fastq -OPTIONS FILES

Detailed information on usage and command line options can be found either by running bcl2fastq --help to see a list of command line options or by referring to the User's Guide.